Covalently trapping MutS on DNA to study DNA mismatch recognition and signaling.
نویسندگان
چکیده
The DNA repair protein MutS forms clamp-like structures on DNA that search for and recognize base mismatches leading to ATP-transformed signaling clamps. In this study, the mobile MutS clamps were trapped on DNA in a functional state using single-cysteine variants of MutS and thiol-modified homoduplex or heteroduplex DNA. This approach allows stabilization of various transient MutS-DNA complexes and will enable their structural and functional analysis.
منابع مشابه
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عنوان ژورنال:
- Molecular bioSystems
دوره 8 7 شماره
صفحات -
تاریخ انتشار 2012